P-27: Assessment of the Contraceptive Effects of Cyclo Hexyl and Propylene Glycol on Histological Changes of Rat Seminiferous Tubules

Background: According to highlighted role of men in family planning, the use of reversible methods of contraception with no adverse effects on androgen-dependent metabolic reactions is very important. Therefore, in order to achieve a conductor combination in the designing contraceptive drugs, in the study, the effect of two drugs: Propylene glycol and Cyclo Hexyl on histological parameters of seminiferous tubules was investigated. Materials and Methods: This study was conducted on the 33 rat Sprogue-Dawley with weighing 250-300 g and least 100 days. Then they were divided into a group containing 13 mice and two groups containing 10 mice and were Received respectively 15 mg/kg of Propylene glycol and Cyclo Hexyl and normal saline as subcutaneous injection daily for 60 days. Next, samples of testicular fixative were stained by the usual method (H&E) and Histological parameters of seminiferous tubules were measured through stereometry and morphometry methods. Results: Reduce the Diameter and Perimeter of seminiferous tubules in the group of Cyclo Hexyl had significant difference with the two other groups while there was not noticeable difference among the three studied groups in terms of seminiferous tubules number. Conclusion: Cyclo Hexyl was more effective on Histological parameters of seminiferous tubules. Thus with eliminating the cardiovascular effects and Conservation the contraceptives properties, It can be used as a guide combination.